Products

Highly Competitive Performance - High Throughput, Excellent Sample Preservation Rate & Detection Sensitivity

DNA polymerase Ⅰ Klenow Fragment(5U/μL)

5U/μL

Stored at: -20℃

    REF    GSP9161        Spec: 50μL(250U)

                 GSP9162                  100μL(500U)

                 GSP9163                  500μL(2500U)

                 GSP9164                  1000μL(5000U)



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DNA polymerase Ⅰ Klenow Fragment(5U/μL)

Klenow Fragment is a mesophilic DNA polymerase derived from the E. coli Polymerase I. The enzyme exhibits DNA synthesis and proofreading (3′→5′) nuclease activities, and, in the absence of the holoenzyme’s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis.


Features 
  • Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for  sequencing

  • High fidelity, strong chain switching activity, suitable for whole genome amplification

Applications
  • Cut off the 3 'protruding end or flatten the 5' protruding end to form a flat end

  • Preparation of probes or markers using random primers

  • Synthesis of double stranded DNA in oligonucleotide directed mutagenesis

  • Double deoxygenation method for DNA sequencing (Sanger method)

Performance

1. The enzyme synthesis activity is consistent with well-known domestic brands


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Fig. DNA extends, fragment length increases, and Tm value increases


2. Excellent database connection performance after flat end construction


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Fig. Plasma free DNA was subjected to flat end ligation and then a library was constructed