Highly Competitive Performance - High Throughput, Excellent Sample Preservation Rate & Detection Sensitivity
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules.T4 PNK also exhibits 3′-phosphatase and 2′,3′ cyclic phosphodiesterase activities.
5'-phosphorylation of oligonucleotides, PCR products, other DNA or RNA prior to ligation;
Phosphorylation of PCR primers;
Detection of DNA modification by the [32P]-postlabeling assay;
Removal of 3'-phosphate group