Highly Competitive Performance - High Throughput, Excellent Sample Preservation Rate & Detection Sensitivity
LbCas12a was purified from E. coli strain containing the Lachnospiraceae bacterium CRISPR-Cas12a expression gene. LbCas12a belongs to V-type CRISPR Cas system. In the presence of PAM in the target DNA, it is a RNA mediated Nuclease that specifically targets to cut double stranded DNA. Compared to Cas9, Cas12a only requires a single crRNA and has a smaller volume, making it easier to deliver into cells; At the same time, the combination of LbCas12a crRNA complex with complementary single stranded DNA or double stranded DNA releases strong non-specific ssDNA trans cleavage activity, which can be used for Genetic testing and imaging.
Multiple enzymes work together at room temperature to achieve rapid amplification of nucleic acids, which is a true POCT technology. It is a next-generation high sensitivity, high specificity, fast isothermal Nucleic acid test technology based on CRISPR detection development
1. Excellent enzyme activity
Fig. The enzyme activities of LbCas12a produced by Ardent far exceed those of world-class companies
2. Excellent enzyme stability
Fig. The fluorescence results of LbCas12a showed no significant difference compared to those after 0 hours of storage, indicating that the LbCas12a enzyme produced by Ardent has excellent stability
