Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The  AP-lyase activity cleaves both 3′ and 5′ to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine,methy-fapy-guanine,  fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.

1. The enzyme activity of Ardent and well-known foreign company N is consistent

Fig. If there is no Fpg, the primers will complement each other with a high Tm value. If there are Fpg cutting primers, the primers will not complement each other and the Tm value is low.