This product originates from E.coli and is involved in DNA damage repair. This enzyme can recognize the depurine/depyrimidine  (AP) sites on double stranded DNA molecules and cleave the first phosphate diester bond at the 5' end of the AP site, producing 3'  hydroxyl and 5' deoxyribose phosphate terminals; In addition, endonuclease IV also has 3' diesterase activity, which can release phosphoglyceraldehyde, intact deoxyribose 5' - phosphate, and phosphate from the 3 'end of DNA. This enzyme also has 3' -5 'exonuclease activity, and its progressive action on the substrate is influenced by metal ions, DTT, EDTA, etc., and is more inclined to act on double stranded DNA at the 3' concave end.

1. Enzyme activity is basically consistent with well-known domestic brands

Fig. A constant temperature rapid amplifcation reaction was performed, and the target fragment could be amplified with two blurry bands of different sizes. The small bands are generated under the action of Nfo cutting

2. Reaction between Ardent Nfo IPA products and various test strips

Fig. The product cut by Nfo reacted on the test strip, and the T-line showed a positive result