Highly Competitive Performance - High Throughput, Excellent Sample Preservation Rate & Detection Sensitivity
This kit is a real-time fluorescence amplification detection reagent for isothermal nucleic acid based on IPA technology.
The target DNA product can be amplified at a constant temperature (39-42℃). When the probe modified by THF is combined
with the target DNA product, Exonuclease Ⅲ in the reagent recognizes the site, and the THF residue is removed, resulting in
the separation of the fluorescent group and the quenching group to emit a fluorescence signal. The real-time fluorescence
curve can be obtained by a fluorescence PCR instrument.
High sensitivity: IPA can detect a small amount of DNA in the sample;
Fast reaction speed: at the reaction temperature of 39~42℃, it only takes 30 minutes to amplify to a detectable level, but it also depends on the size of the target fragment and the template concentration;
Strong specificity: it can identify and amplify a single DNA molecule from a sample containing multiple unrelated compound genomic DNA, and has strong anti-interference ability;
Can be made into freeze-dried state, and has simple operation and easy storage.
