Highly Competitive Performance - High Throughput, Excellent Sample Preservation Rate & Detection Sensitivity
Name | Method | Specifications | Storage | Valid period |
Nucleic Acid Extraction or Purification Kit | Column Filtering Bead Binding | 20 tests/Kit,40 tests/Kit | RT | 18 months |
20 tests/Kit,50 tests/Kit |
Nucleic Acid Extraction or Purification Kit(Plasma cfDNA)
● Introduction
This product is based on the extraction of cfDNA from plasma using either the Column Filtering or Bead Binding. The sample is then subjected to lysis digestion, adsorption binding, washing to remove impurities, and low salt buffer elution to collect DNA. The washed DNA can be directly used for experiments such as PCR, Southern blot, and virus detection.
● Features
Large-capacity extraction: Concentrated nucleic acids, with high input(4-10 mL plasma)and low elution volumes
High nucleic acid yield: Efficient recovery of fragmented DNA(more than 80%)
High sensitivity: Sufficient amount of template can effectively ensure product concentration and improve detection sensitivity
Convenient and flexible: Simple operation, flexible selection of different operation methods (Column Filtering/Bead Binding)
● Applications
Noninvasive prenatal screening
Early screening of tumours
Cardiovascular disease
Transplant rejection
● Performance
Column Filtering
High-yield cfDNA
Fig. There is no difference in cfDNA yield between Ardent and Q Company
Efficient column filtering, saving experiment time
Fig. The column time of Ardent kit is significantly better than that of famous Q company
Efficient recovery of short DNA fragments
Fig. After building the template library obtained by extraction reagents between Ardent and internationally known Q company, electrophoresis using QSP400 shows that the peaks of interest can coincide well and without difference
cfDNA recovery rate is over 80%
Fig. The recovery rate of all different fragments above 50 bp can reach more than 80%
Bead Binding
High short fragment recovery rate
Fig. Using the Ardent extraction kit to extract cfDNA from 4mL of plasma samples, the recovery rate of fragments (over 75bp) reached over 90%
The extraction effect of Ardent extraction kit is basically consistent with that of Q brand