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Nucleic Acid Extraction or Purification Kit(Serum, plasma cfDNA)

NameMethodSpecificationsStorageValid period
Nucleic Acid Extraction or 

Purification Kit

Column Filtering

Bead Binding

20 tests/Kit,40 tests/KitRT18 months
20 tests/Kit,50 tests/Kit


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Nucleic Acid Extraction or Purification Kit(Plasma cfDNA)


  Introduction


This product is based on the extraction of cfDNA from plasma using either the Column Filtering or Bead Binding. The sample is then subjected to lysis digestion, adsorption binding, washing to remove impurities, and low salt buffer elution to collect DNA. The washed DNA can be directly used for experiments such as PCR, Southern blot, and virus detection.


  Features


  • Large-capacity extraction: Concentrated nucleic acids, with high input(4-10 mL plasma)and low elution volumes

  • High nucleic acid yield: Efficient recovery of fragmented DNA(more than 80%)

  • High sensitivity: Sufficient amount of template can effectively ensure product concentration and improve detection sensitivity

  • Convenient and flexible: Simple operation, flexible selection of different operation methods (Column Filtering/Bead Binding)


  Applications


  • Noninvasive prenatal screening

  • Early screening of tumours

  • Cardiovascular disease

  • Transplant rejection


  Performance


Column Filtering

  • High-yield cfDNA

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Fig. There is no difference in cfDNA yield between Ardent and Q Company


  • Efficient column filtering, saving experiment time

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Fig. The column time of Ardent kit is significantly better than that of famous Q company


  • Efficient recovery of short DNA fragments

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Fig. After building the template library obtained by extraction reagents between Ardent and internationally known Q company, electrophoresis using QSP400 shows that the peaks of interest can coincide well and without difference


  • cfDNA recovery rate is over 80%

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Fig. The recovery rate of all different fragments above 50 bp can reach more than 80%


Bead Binding

  • High short fragment recovery rate

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Fig. Using the Ardent extraction kit to extract cfDNA from 4mL of plasma samples, the recovery rate of fragments (over 75bp) reached over 90%


  • The extraction effect of Ardent extraction kit is basically consistent with that of Q brand 

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