LbuCas13a was purified from E. coli strain containing the expression gene of Leptotrichia buccalis CRISPR-Cas13a. LbuCas13a is a type VI CRISPR Cas system, which is a RNA mediated Nuclease targeting RNA cleavage. LbuCas13a has high specificity and sensitivity, and can be used to detect individual RNA molecules. The Nobel Prize winner Doudna team has established a tandem CRISPR Nuclease system based on this, which has a broad application prospect in the field of Nucleic acid test.

• Multiple enzymes work together at room temperature to achieve rapid amplification of nucleic acids, which is a true POCT technology. It is a next-generation high sensitivity, high specificity, fast isothermal Nucleic acid test technology based on CRISPR detection development